The amiRNA Designer web app designs artificial microRNA to target one or multiple genes in different eudicot and monocot plant species. The app outputs the sequence of an amiRNA together with the sequence of the two oligonucleotides required for cloning the amiRNA in BsaI-ccdB (B/c) vectors containing the Arabidopsis thaliana MIR390a or the Oryza sativa MIR390 foldbacks for use in eudicot or monocot species, respectively. Multiple results are provided when possible. If you already have an amiRNA sequence, the app directly outputs the sequence of the oligonucleotides. amiRNA Designer can filter amiRNA for potential off-targets if you want a highly specific amiRNA.Get Started.