LOCUS       pENTR-AtTAS1c-B/c_AC    4989 bp      DNA     circular   6/4/14
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     ncRNA           689..1108
                     /label=AtTAS1c 5' region
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    1110..1115
                     /label=BsaI site
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     CDS             1216..1875
                     /label=Choramphenicol resistance gene
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_recomb     569..668
                     /label=AttL1
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      complement(427..470)
                     /label=rrnB T1
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      complement(268..295)
                     /label=rrnB T2
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     rep_origin      4317..4945
                     /label=col1 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    2563..2568
                     /label=Inverted BsaI site
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     CDS             2217..2522
                     /label=ccdB gene
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     ncRNA           2570..3097
                     /label=AtTAS1c 3' region
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_recomb     complement(3114..3213)
                     /label=AttL2
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     primer_bind     complement(3230..3270)
                     /label=M13-R
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     promoter        complement(3230..3249)
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      4317..4945
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     CDS             3383..4189
                     /label=Kanamycin resistance gene
                     /SerialCloner_Color=&h996633
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     primer_bind     536..553
                     /label=M13-F
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 
      661 AGCAGGCTCC GCGGCCGCCC CCTTCACCAA ACCTAAACCT AAACGGCTAA GCCCGACGTC 
      721 AAATACCAAA AAGAGAAAAA CAAGAGCGCC GTCAAGCTCT GCAAATACGA TCTGTAAGTC 
      781 CATCTTAACA CAAAAGTGAG ATGGGTTCTT AGATCATGTT CCGCCGTTAG ATCGAGTCAT 
      841 GGTCTTGTCT CATAGAAAGG TACTTTCGTT TACTTCTTTT GAGTATCGAG TAGAGCGTCG 
      901 TCTATAGTTA GTTTGAGATT GCGTTTGTCA GAAGTTAGGT TCAATGTCCC GGTCCAATTT 
      961 TCACCAGCCA TGTGTCAGTT TCGTTCCTTC CCGTCCTCTT CTTTGATTTC GTTGGGTTAC 
     1021 GGATGTTTTC GAGATGAAAC AGCATTGTTT TGTTGTGATT TTTCTCTACA AGCGAATAGA 
     1081 CCATTTATCG GTGGATCTTA GAAAATTAAG AGACCATTAG GCACCCCAGG CTTTACACTT 
     1141 TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG AGCCGTCGAG ATTTTCAGGA 
     1201 GCTAAGGAAG CTAAAatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 
     1261 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 
     1321 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 
     1381 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg 
     1441 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 
     1501 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 
     1561 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 
     1621 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 
     1681 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 
     1741 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt 
     1801 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 
     1861 cagggcgggg cgtaaACGCG TGGAGCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT 
     1921 TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA 
     1981 AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT 
     2041 TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA 
     2101 AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT 
     2161 CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC 
     2221 AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA 
     2281 GTGATATTAT TGACACGCCC GGCCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC 
     2341 TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC 
     2401 GCATGATGAC CACCGATATG GCCAGTGTGC CGGTTTCCGT TATCGGGGAA GAAGTGGCTG 
     2461 ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT 
     2521 AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCACCTCG ACggtctcAg aactagaaaa 
     2581 gacattggac atattccagg atatgcaaaa gaaaacaatg aatattgttt tgaatgtgtt 
     2641 caagtaaatg agattttcaa gtcgtctaaa gaacagttgc taatacagtt acttatttca 
     2701 ataaataatt ggttctaata atacaaaaca tattcgagga tatgcagaaa aaaagatgtt 
     2761 tgttattttg aaaagcttga gtagtttctc tccgaggtgt agcgaagaag catcatctac 
     2821 tttgtaatgt aattttcttt atgttttcac tttgtaattt tatttgtgtt aatgtaccat 
     2881 ggccgatatc ggttttattg aaagaaaatt tatgttactt ctgttttggc tttgcaatca 
     2941 gttatgctag ttttcttata ccctttcgta agcttcctaa ggaatcgttc attgatttcc 
     3001 actgcttcat tgtatattaa aactttacaa ctgtatcgac catcatataa ttctgggtca 
     3061 agagatgaaa atagaacacc acatcgtaaa gtgaaatAAG GGTGGGCGCG CCGACCCAGC 
     3121 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 
     3181 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 
     3241 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 
     3301 ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 
     3361 AAACAGTAAT ACAAGGGGTG TTatgagcca tattcaacgg gaaacgtcga ggccgcgatt 
     3421 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 
     3481 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 
     3541 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 
     3601 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 
     3661 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 
     3721 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 
     3781 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 
     3841 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 
     3901 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 
     3961 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 
     4021 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 
     4081 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 
     4141 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttcT AATCAGAATT 
     4201 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 
     4261 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 
     4321 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 
     4381 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 
     4441 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 
     4501 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 
     4561 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 
     4621 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 
     4681 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 
     4741 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 
     4801 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 
     4861 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 
     4921 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 
     4981 TCACATGTT
//