The Plant Small RNAMaker Site (P-SAMS)
The P-SAMS web application is no longer maintained, but P-SAMS is still available as a standalone tool. The standalone version of P-SAMS is available on GitHub.
Contact
For any questions, please contact us.
Methods
Software
P-SAMS software is open-source and is available on GitHub.
How to cite
If using P-SAMS please cite:
Fahlgren, N, Hill ST, Carrington JC, Carbonell A (2016) P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design. Bioinformatics 32: 157-158. doi: 10.1093/bioinformatics/btv534.
If using AtMIR390a- or AtTAS1c-based B/c vectors please cite:
Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC (2014) New generation of artificial microRNA and synthetic trans-acting small interfering RNA vectors for efficient gene silencing in Arabidopsis. Plant Physiology 165: 15–29. doi: 10.1104/pp.113.234989.
If using OsMIR390-based B/c vectors please cite:
Carbonell A., Fahlgren N, Mitchell S, Cox Jr KL, Reilly KC, Mockler TC, Carrington JC (2015) Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors. The Plant Journal 82: 1061-1075. doi: 10.1111/tpj.12835.
Acknowledgements
This work was supported by grants from the National Science Foundation (MCB-0956526, MCB-1231726) and National Institutes of Health (AI043288) to James C. Carrington, and fellowship from the United States Department of Agriculture (National Institute of Food and Agriculture, 2012-67012-19899) to Noah Fahlgren. We thank the Donald Danforth Plant Science Center Data Science Core Facility for web development and hosting services and the Carrington lab members for useful suggestions and beta-testing.
License
The MIT License (MIT)
Copyright (c) 2024 Donald Danforth Plant Science Center
Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.